Work packages

Spectrometry and chemometry

Raman, NIR, MIR, 2D fluorescence and chemometry support

One of the analytical technologies investigated for the integrated panel (PAOLA) are spectrometry and chemometry based detection methods. The purpose of this WP is to investigate Raman, NIR, MIR and 2D fluorescence spectroscopy for the detection and quantitation of varying parameters consisting of, but not limited to, metabolites, amino acids, (viable) cell concentration, titre, CQA attributes, viability and bioburden in various cell culture processes (fed-batch and continuous) in order to provide validated tools for monitoring and adjusting (control-loop) the processes in (near) real-time. 


Introduction

The combination of spectrometry and chemometry tools can be applicable for development purposes as well as for the implementation in GMP processes. Knowledge and experience from academic and industrial partners will be applied during the development as well as close interaction with the following work packages:

WP 2: High Through put micro-bioreactors (transfer from small scale to production scale)

WP 4: Optical on-line sensors (for supplementary information)

WP 7: Sampling preparation (e.g. cell free sampling for at line analytics)

WP 8: Models Ð data driven, mechanistic, control




Workpackage objectives 

  • Identify spectroscopic direct and indirect measurable process variables
  • Determine spectral pre-processing procedures
  • Determine meaningful wavelengths (combinations) for modelling
  • Develop chemometric models methodology
  • Module for optimal design of experiment WP2
  • Determine the transferability of chemometric models (MBR to STR)
  • Define validation procedures of chemometric models for life cycle management
  • Contribution of chemometric models for closed-loop control
  • Select spectrophotometric method (Raman, NIR, MIR, 2D fluorescence, or wavelength combinations) to be used.

Achievements

Spectral library from bioreactor cultivations constructed


Leader and co-leader

UH

Sanofi


Partners

UMons

KTH, Chotteau

Ipratech

Bayer

Rentschler

Synthon

GSK